HARNESSING SUB-2 μM TECHNOLOGY: THE TRANSFORMATIVE ROLE OF UPLC IN PHARMACEUTICAL ANALYSIS
Hebha Amreen*
Ultra-Performance Liquid Chromatography (UPLC) has emerged as a pivotal advancement in pharmaceutical analysis, leveraging sub-2 μm particle technology to achieve unprecedented speed, resolution, and sensitivity. While traditional High-Performance Liquid Chromatography (HPLC) remains a cornerstone, UPLC’s superior efficiency—governed by the van Deemter equation—enables faster separations with enhanced peak capacity. However, its adoption necessitates careful consideration of operational challenges, including increased backpressure, method scalability, and economic trade-offs. This article critically examines UPLC’s theoretical foundations, practical applications in drug development and quality control, and its integration with mass spectrometry (MS) for bioanalytical assays. We also explore regulatory implications and cost-benefit analyses to guide strategic implementation in pharmaceutical laboratories.
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