PHARMACEUTICAL EVALUATION BY DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF ATENOLOL IN PHARMACEUTICAL DOSAGE FORM
Dr. Fatehalrahman F. Magbool*, Mahmoud A. Ibrahim and Somia E. Ahmed
Objective: In this study, a simple, low cost, sensitive, highly accurate and precise ultraviolet spectrophotometric method has been developed and validated for estimation of Atenolol (ATN) in tablets pharmaceutical dosage form, ATN show maximum absorbance at 274 nm in mixture of methanol water 1:9 v/v, the methods was linear in the range 0.06-0.2 mg/ml. Methods: The method obtained with low cost term by using distilled water (DW) as major solvent 99% ATN absorbance showed linearity in the concentration range of 0.06- 0.2 mg/ml with correlation coefficient 0.999, intercept equal to 0.0015, detection limit was found (0.00375mg/ml), quantification limit was (0.03 mg/ml), which proves suitability of proposed method for determination of ATL in pharmaceutical tablets dosage form according to the statistical analysis of the results due to high accuracy and precession. Results: The relative standard deviations value for accuracy and precisions were less than 0.095 with repeatability value between 103.38- 100.89. Conclusions: The developed method is simple, low cost, sensitive, highly accurate and precise and easily can be used for estimation of Atenolol in pharmaceutical dosage form.
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